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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene has actually been observed in many tumors, which leads to chromosome instability, and therefore may perhaps add to tumorigenesis. This protein was uncovered to affiliate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in mobile-cycle controlled histone gene expression and performs a essential function in promoting mobile-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are described. Two more splice variants were described but in depth nucleotide sequence information will not be however accessible.

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Blocking peptides are peptides that bind specifically on the focus on antibody and block antibody binding. These peptide typically consists of the epitope acknowledged by the antibody.

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This guidebook outlines even more probable optimization of mobile and immunoassay parameters to make sure the absolute best outcomes are...

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This guidebook outlines even more attainable optimization of cellular and immunoassay parameters to make certain the best possible outcomes are...

Antibodies bound to the blocking peptide now not bind into the epitope over the concentrate on protein. By evaluating the staining with the blocked antibody versus the antibody by itself, you can see which staining is particular.

Focus on Information and facts Cyclin E belongs on the very conserved cyclin family members, whose users are characterised by a remarkable periodicity in protein abundance with the cell cycle. Cyclins perform as regulators of CDK kinases. Diverse cyclins show distinct expression and degradation designs which add on the temporal coordination of each and every mitotic function. Cyclin E sorts a fancy with and capabilities as being a regulatory subunit of CDK2, whose action is necessary for mobile cycle G1/S transition. This protein accumulates with the G1-S section boundary and is degraded as cells progress by S section.

The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...

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